Journal: Drug Delivery

Article Title: The colloidal stability of albumin-based drug delivery systems has a profound effect on tumoricidal activity

doi: 10.1080/10717544.2026.2614801

Figure Lengend Snippet: In vitro cytotoxicity of HSA-PLA (PTX) and Abraxane in human cancer cell lines. Data are presented as mean ± SD ( n = 3 independent experiments per group). Data for HSA-PLA (PTX) were adapted from our previous publication (Xiong et al. ).

Article Snippet: The murine breast cancer cell line (4T1, CRL-2539), human breast cancer cell line (BT-549, HTB-122), human prostate cancer cell line (PC-3, CRL-1435), and human pancreatic cancer cell line (MIA PaCa-2, CRM-CRL-1420) were obtained from stocks originally purchased from ATCC (UK).

Techniques: In Vitro

Journal: Cancer Pathogenesis and Therapy

Article Title: Neuroendocrine prostate cancer (NEPC)-associated CEP55 promotes cisplatin resistance in prostate cancer by regulating CDK1 phosphorylation

doi: 10.1016/j.cpt.2025.06.008

Figure Lengend Snippet: CEP55 plays an oncogenic role in prostate cancer. (A) The transcript levels of CEP55 in 22 paired prostate tumors and adjacent tissues. (B) The protein level of CEP55 in 4 paired prostate tumors and adjacent tissues. (C) The immunohistochemical picture of 2 paired prostate tumors and adjacent tissues. The scale on the lower left corner represented 100 μm. (D) The protein level of CEP55 in Lncap, C4-2, DU145, and PC3 cells. (E) The translation validation after CEP55 overexpression and knockdown in PC3 cells. (F–K) Cell viability, clone formation, migration, and analysis about DU145, PC3 cells after CEP55 knockdown (F–H) and overexpression (I–K). The scale on the lower right corner represented 200 μm. (L) Subcutaneous tumor formation in nude mice after injecting 1×10 7 shNC or sh CEP55 -1 PC3 cells. The tumor volume was measured every week. Data are represented as mean ± SD. (M) The protein levels of CEP55 , CHGA , NSE , and SYP after CEP55 overexpression in DU145, PC3 cells. (N) The protein levels of CEP55 , CHGA , NSE , and SYP after CEP55 knockdown in PC3 cells. GAPDH acted as an internal reference. ns-not significant difference, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. CEP55 : Centrosomal protein 55; sh CEP55 : Knockdown of CEP5 5 by shRNA; oeCEP55: Overexpression of CEP55; si CEP55 : Small interfering RNA targeting CEP55 ; A 450 : Optical Density at 450 nm; CHGA : Chromogranin A gene; NSE : Neuron-Specific Enolase; SYP : Synaptophysin.

Article Snippet: Prostate cancer cell lines PC3, DU145, C4-2, LNcap, and human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (ATCC).

Techniques: Immunohistochemical staining, Biomarker Discovery, Over Expression, Knockdown, Migration, shRNA, Small Interfering RNA

Journal: Cancer Pathogenesis and Therapy

Article Title: Neuroendocrine prostate cancer (NEPC)-associated CEP55 promotes cisplatin resistance in prostate cancer by regulating CDK1 phosphorylation

doi: 10.1016/j.cpt.2025.06.008

Figure Lengend Snippet: CEP55 mediated cisplatin resistance via CDK1 phosphorylation at Tyr15 in prostate cancer cells. (A) Relative cell survival rate after CEP55 knockdown and overexpression in DU145 and PC3 cells treated with 1 μg/mL cisplatin for 24 h or not. (B–D) GSEA, GO, and KEGG analyses after being divided into low- and high- CEP55 expression groups from the TCGA database. (E) KEGG analysis of RNA sequencing in shNC and sh CEP55 -2 PC3 cells. (F) Cell cycle assessment of in shNC, sh CEP55 DU145, and PC3 cells treated with 1 μg/mL cisplatin for 24 h. (G) Analysis of co-immunoprecipitation between CEP55 and phospho-CDK1 Tyr15 in DU145, PC3 cells. The IgG group acted as a negative control. (H) The protein level of CEP55 , CDK1, and phospho-CDK1 Tyr15 in shNC, sh CEP55 -2 DU145 cells treated with 1 μg/mL cisplatin for 0, 8, 16, and 24 h. Data are represented as mean ± SD. ns-not significant difference, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. CEP55 : Centrosomal protein 55; sh CEP55 : Knockdown of CEP5 5 by shRNA; oeCEP55: Overexpression of CEP55; si CEP55 : Small interfering RNA targeting CEP55 ; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; GSEA: Gene Set Enrichment Analysis; CDK1: Cyclin-dependent kinase 1; Tyr15: Tyrosine 15.

Article Snippet: Prostate cancer cell lines PC3, DU145, C4-2, LNcap, and human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (ATCC).

Techniques: Phospho-proteomics, Knockdown, Over Expression, Expressing, RNA Sequencing, Immunoprecipitation, Negative Control, shRNA, Small Interfering RNA